Protocol for purifying biologically active microtubule severing protein UNC 45A from E.coli using GST affinity and spin columns: STAR Protocols CMTM3 Promotes Colitis associated Carcinogenesis via CLTC Stabilization and Modulation of VE cadherin Cellular and Molecular Gastroenterology and Hepatology Glutathione S transferase (GST) Gene Fusion System Metabolic profiling reveals pyrimidine synthesis enzyme CAD as a central carbon metabolism signaling node in cancer cell proliferation ScienceDirect Structural basis of mRNA binding by the human FERRY Rab5 effector complex: Molecular Cell